Former Mexican federal agent Machete (Danny Trejo) ignored his superiors' orders and insisted on tracking down the criminal boss Trey (Steven Seagal), but was assassinated, resulting in the murder of his wife and daughter. Three years later, Machete worked to make a living in Texas near the Mexican border. By chance, he was spotted by a mysterious figure (Jeff Fahey) and hired to assassinate Senator John McLaughlin (Robert DeNiro), who promoted a tough immigration policy, but Machete was assassinated again during the action and began to be hunted down by his employer and Trey behind the scenes. In a critical moment, Machete was helped by Luz (Michelle Rodriguez), female immigration agent Shatana (Jessica Alba) and many friends. He gradually realized that there was an ugly conspiracy behind all this that mixed politics and drugs, and a just revenge began...